Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy
نویسندگان
چکیده
Digital holographic microscopy provides the ability to observe throughout a volume that is large compared its resolution without need actively refocus capture entire volume. This enables simultaneous observations of numbers small objects within such We have constructed microscope can 0.4 µm × 1.0 with submicrometer (in xy) and 2 z) for observation microorganisms minerals in liquid environments on Earth potential planetary missions. Because environmental samples are likely contain mixtures inorganics comparable sizes near limit instrument, discrimination between living non-living may be difficult. The active motion motile organisms used readily distinguish them from non-motile (live or inorganic), but additional methods required inorganic size different composition structure. demonstrate use passive make this by evaluating diffusion buoyancy characteristics cells, styrene beads, alumina particles, gas-filled vesicles micron scale field view.
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ژورنال
عنوان ژورنال: Life
سال: 2021
ISSN: ['2075-1729']
DOI: https://doi.org/10.3390/life11080793